sspI [2019-07-09 08:05:01]

small acid-soluble spore protein (minor), SASP

locus: BSU28660

pI: 4.49

mw: 7.72 kDa

protein length: 71 aa

gene length: 216 bp

function: protection of spore DNA

product: small acid-soluble spore protein (minor), SASP

essential: no

synonyms: ysfA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.3|Small acid-soluble spore proteins]

Gene

Coordinates: 2,931,692 2,931,907

The protein

Protein family

• sspI family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6E0B963D2334CEAFC19E4B6E224F7899A41A4B94|sspI]
  description: [Pubmed|10806362]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325,10806362], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325,10806362]

additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

Biological materials

Mutant

• MGNA-B025 (ysfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1024 NBRP B. subtilis, Japan]
• BKE28660 ([gene|6E0B963D2334CEAFC19E4B6E224F7899A41A4B94|sspI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACGCTCCTTTTT, downstream forward: _UP4_TAAGACATGAAACCGGGTGA
• BKK28660 ([gene|6E0B963D2334CEAFC19E4B6E224F7899A41A4B94|sspI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACGCTCCTTTTT, downstream forward: _UP4_TAAGACATGAAACCGGGTGA

References

• 10806362,16497325,30782632